The roles and responsibilities of women increase after they become mothers. Because of the increase in their roles and responsibilities, the mothers can experience difficulties from time to time, and these can lead to mental disorders. One of the factors that have effect on this is working status of mothers. While there are many researches about the impact of the mothers’ working status on children, the number of studies about the relationship between maternal work and maternal mental health status is relatively small. The aim of this study is to investigate the relationship between the levels of depression, anxiety and working status of mothers. The study sample consisted of 73 non-working and 70 working mothers with children under 5 years of age. The Edinburgh Postnatal Depression Scale, Beck Anxiety Inventory, Pharis Self-Confidence Scale, and Maternal Attachment Inventory and two different sociodemographic data forms according to working status were used. Despite the lack of statistical significance in relation to depression cut-off score and working status according to EPDS, depression was found to be numerically less frequent in working mothers. According to the BAI, those with moderate to high anxiety were observed to be more frequent among non-working mothers. PMS and MAI scale scores were found to be similar in both groups. Although there are many different factors that may affect these findings it is possible to conclude that having a job has positive effects on mothers’ mental health. Depending on these findings, it can be suggested that the support of mothers can be increased to encourge them to have a working life.